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The Effect and Mechanisms of FasL Gene-Modified Dendritic Cells Alleviating GVHD
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Abstract:
Objective：To alleviate graft versus host disease(GVHD) via depletion of alloreactive cells from haematopoietic stem cell grafts. Methods：Balb/c mice dendritic cells genetically engineered to express FasL were cultured with C57BL/6 mice stem cell grafts, and the modified stem cell grafts were used in a C57BL/6 to Balb/c mice GVHD model system (H-2b→H-2d). Then the GVHD clinical manifestations(diarrhea, depilate, lymphocytes infiltration in target tissues) were observed and compared.Results：Recipients that received donor haematopoietic stem cell grafts pretreated with FasL-DC did not develop lethal GVHD, and their survival was also surprisingly prolonged. In contrast, recipients receiving untreated allogeneic grafts displayed all clinical signs of acute GVHD, and died within 30 days after transplantation.Conclusion：DC transfected with FasL gene can prevent GVHD by selective removal of alloreactive cells from haematopoietic stem cell grafts.
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Clc Number:
R730.5 R979.1
