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The Expression and Protective Efficacy of DNA Vaccine Encoding Antibodized hgp100
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Abstract:
Objective:To investigate whether the plasmid γ1neo-hgp100 could be expressed and presented in vitro and could protect the immunized mice from B16F10 challenge in vivo.Methods: γ1neo-hgp100 plasmid was constructed in which the DNA sequence encoding hgp100 CTL epitope inserted into CDR3 of γ1-neo vector. The expression of antibodized antigen and IFN-γ in supernatant was measured by ELISA respectively after transfection J558L with γ1neo-hgp100 and further co-culture of J588L transfacted with γ1 neo-hgp100 and pmel TCR transgenic T cell. After introspleenic inoculation of γ1neo-hgp100, the protective efficacy of the gene vaccine was observed by means of measuring the tumor area every two days. Results: γ1neo-hgp100 could be expressed and presented in vitro, the immunogenecity of CTL epitope of hgp100 was strong enough and could activate gp100 specific T cell, the mice immunized with the gene vaccine could resist the tumor challenging in vivo. The mean survival time was prolonged to 36 days, compared to control group(P<005). Conclusion: γ1neo-hgp100 could be expressed and presented in vitro and protect mice from tumor challenging.
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Clc Number:
R735.34 R737.25
