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Inhibition of fat-1 Gene on Proliferation of Breast Cancer Cells
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Abstract:
Objective: To transfer the gene of n-3 fatty acid desaturase fat-1 into human breast cancer cell QMR2 by adenovirus vector and study the effect of the gene on proliferation of QMR2 cells. Methods: The gene fat-1 was cloned into the shuttle vector of adenovirus, and homologously recombined with an adenoviral backbone vector (pAdEasy 1) to generate the recombinant adenovirus Ad.GFP.fat1; the virus was packaged in 293 cells, inoculated on the breast cancer cells QMR2; total RNA of the cells was hybridized with antisense RNA of fat-1 mRNA by Northern to analyze the expression of fat-1; the effect of fat-1 on the proliferation of QMR2 cells was analyzed by Flow Cytometry; the content of n-6 PUFAs/n-3 PUFAs was analyzed by Gas Chromatography.Results:The high titer recombinant virus was got through DNA recombinant; the fat-1 mRNA appeared in breast cancer cell QMR2 after virus Ad.GFP. fat1 infected the cells for 2 days; compared with the control cells (Ad.GFP), proliferation of QMR2 cells was inhibited by the gene fat-1, decreased by 31%(P<0.05); moreover, fat-1 gene decreased content of n-6 PUFAs/n-3 PUFAs. Conclusion: The gene fat-1 was heterologously expressed in human breast cancer cell QMR2 via adenovirus, and the expression produced an inhibitory effect on proliferation of the cells.
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Clc Number:
R730.2 R735.2
