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The Effect of the Expression Level of CXCR4 on the Metastatic Potential of Human Lung Cancer Cells
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Abstract:
Objective: To study the effect of the expression levels of Chemokine receptor CXCR4 on the metastatic potential of human lung cancer. Methods:CXCR4 expression was determined by Real-time PCR and FACS. The plasmid DNA containing CXCR4 coding gene or CXCR4 antisense nucleotides fragment was constructed and transfected into 95C or 95D cells with LipofectamineTM2000 reagent respectively, and then the stable transfectants were screened by G418. Migratory responses to SDF-1α were detected by chemotaxis and chemoinvasion assay, MMP-2 activity was determined with zymography, and the ability of adhesion to ECV-304 cells was analyzed by FACS. Results: The surface expression of CXCR4 on lung cancer cells was significantly up or down regulated. Following up-regulation of CXCR4 expression on 95C cells, the migratory response to SDF-1α, MMP-2 activity, and the ability of adhesion to ECV-304 cells were consequently enhanced. Conversely, when CXCR4 expression was down regulated on 95D cells, the migratory response to SDF-1α, MMP-2 activity, and the ability of adhesion to ECV-304 cells were significantly impaired. Conclusion: Up or down regulation of CXCR4 expression enhanced or inhibited the metastatic potential of human lung cancer cells, which implied that CXCR4 expression was closely associated with the metastatic potential of human lung cancer cells.
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Clc Number:
R735.1 R996.3
