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Inhibition of Telomerase Activity by Chimeric Oligonucleotide
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Abstract:
Objective: To investigate the inhibitory effect of telomerase activity by chimeric oligonucleotide.Methods: Here various oligonucleotides were designed and synthesized. Inactivity of the telomerase activity inhibitors was observed with HL-60 cell-lysates in vitro and with U-87 clell in vivo. Results: Both phosphorothioate-modified oligonuclotides (PS-ODNs) and oligonucleotides complementary with telomerase RNA template inhibited telomerase activity. And application of the tow ODNs together increased the inhibition activity in upfold. the various modification extensional oligonucleotides complementary with telomerase RNA template at 3′-end of the PS-ODNs enabled the chimeric oligonucleotides to increase their inhibition efficiency. Conclusion: These cODNs emerged as powerful inhibitors of human telomerase detected so for and might be promising candidates to investigate the effect of permanent of inhibition of telomerse on tumor growth.
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