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Cytotoxicity of Newcastle Disease Virus HN Gene on Human Hepatoma Cells SMMC7721
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Abstract:
Objective : To investigate the mechanism on cytotoxicity of human hepatoma cells SMMC7721 induced by NDV HN gene. Methods: 24 h after transfected with the mixture of NDV HN gene and liposome in vitro, SMMC7721 cell mortality rate was measured through MTT staining; the content of cell surface sialic acid was measured; the death pattern of SMMC7721 cells was detected by staining with AO/EB; expressions of HN gene and HLA-A,B,C was measured by FCM. Results: Transfected into SMMC7721 cells 24 h later, pVHN reduced the surface sialic acid of SMMC7721 cells（P<0.05）and killed the cells significantly in vitro; the difference of HN gene expression was significantly between experimental groups and control groups, and the HLA-A,B,C expression was upregulated. Conclusions: The HN gene plays an important role in the reduction of sialic acid of SMMC7721 cells and caused the cell death. The expressions of HN gene was significant and HLA-A,B,C expression was upregulated after HN gene tansfected into SMMC7721 in virto. Therefore, immunogenicity and immunoregonization of tumor cells might be increased, so the SMMC7721 cells was killed.
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