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Adjuvant Effects of CpG Motif-Containing Oligodeoxynucleotide in vitro on Human Esophageal Tumor Vaccine
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Abstract:
Objective:To investigate the adjuvant effects of CpG motif-containing oligodeoxynucleotide (CpG) in vitro on human esophageal cancer vaccine.Methods:Matured dendritic cells (DC) activated by peptides on Eca-109 cell membranes were used for human esophageal tumor vaccine and matured DC activated by peptides on HL-60 cell membranes were used for control vaccine. With adjuvant of CpG or the cell wall skeleton of coryne bacterium parvum (CBPw), we analyzed each group's T-cell proliferation and cytotoxic T lymphocyte (CTL) response. Results:The adjuvant of CpG or CBPw markedly enhanced T-cell proliferation and CTL response（P＜0.01）; The groups with human esophageal tumor vaccine had markedly greater T-cell proliferation and CTL response than other groups（P＜0.05）; The HLA gene locus alleles to match Eca-109 cell gene affected the activity of T cells.Conclusion:CpG may be the optimal adjuvant of human esophageal tumor vaccine and the HLA gene locus may affect T cells' function.
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