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The Therapentic Effect of Influenza A Virus on Murine S180 Ascites Tumor
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Abstract:
Objective:To investigate the immunological mechanism of influenza A virus for murine S180 ascites sarcoma.Methods:After inocutation with S180 sarcoma cells, mice were i.p. injected with influenza A virus or vehicle 15 days. The average living time and survival rate of the mice were examined. The levels of IL-2, IL-6 and TNF-α were detected. The sarcoma cell′s apoptosis was detected by DNA ladder, flow cytometry (FMC), fluorescent microscope and electron microscope (EM). Results: The average living time and survival rate of the mice injected with Influenza A virus were significantly longer or higher than that of the controls. The levels of IL-2, IL-6 and TNF-α also had the same differences. The apoptosis cells were detected by EM and fluorescent microscope. Sub-diploid peaks were observed by FCM analysis and DNA ladder was seen after electrophoresis in the ascites cells. Conclusion:Our results demonstrated that the feasibility and potential of delivery of influenza A virus as a general means for the treatment of S180 ascites sarcoma.
Keywords:
influenza A virus ; S180 ascites sarcoma ; cytokine ; apoptosis
