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Immunological Mechanism of Mouse Syngeneic Graft Versus Host Disease Model and Its Significance
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Abstract:
Objective:To primarily explore the immunological mechanism of SGVHD and provide a platform for study of graft-versus leukemia.Methods: Lethally irradiated C3H/HeJ mice were reconstructed with syngeneic bone marrow cells and induced with cyclosporine (CsA) for 21d. After cessation of CsA , the clinical symptoms of SGVHD(such as weight loss, diarrhea, hunching, runting) were observed. The histological changes of murine liver, colon, skin, thymus and ear were examined by routine pathological methods. Meanwhile, the expression of cytokines in mice colon and liver were examined by RT-PCR. Results: 67% (19/28) of mice developed GVHD-like clinical symptom in SGVHD-induced group and their pathological detection of liver and colon showed tissue necrosis and increased lymphocyte infiltration .The levels of costimulatory molecule (CD137) and Th1-type cytokines（IL-12, IFN-γ and TNF-α）mRNA were enhanced in liver and colon of SGVHD mice, compared with control mice.Conclusion: The CsA can induce syngeneic BMT recipients of C3H/HeJ mice to develop SGVHD, and costimulatory molecules and Th1 type cytokines played an important role in development of SGVHD.
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