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The Inhibitory Effect of 67 kD LN-R Antisense Oligonucleotides on Invasion and Metastasis of Ovarian Carcinoma Cells
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Abstract:
Objective:To investigate the effect of 67 kD laminin receptor antisense oligonucleotides (67kD LN-R ASODN) on the invasion and metastasis abilities of ovarian carcinoma cell line HRA.Methods:The ASODN of 67 kD laminin receptor and its control, sense oligonucleotides (SODN) were synthesized and transfected into the target cells. RT-PCR and FCM methods were performed to evaluate the 67 kD LN-R gene and protein expression levels to identify the efficiency of ASODN. The invasiveness of transfected cells was measured quantitatively by matrigel invasion assays (transwell chamber). Results: The 67 kD LN-R gene and protein expression and invasiveness of HRA cells treated with ASODN of different final concentration were significantly decreased compared with that transfected with SODN and the controls（P<0.05）. Conclusion:67 kD LN-R ASODN has a significant inhibitory effect on the invasiveness of human ovarian carcinoma cell line HRA in a dose-dependent manner. It may become a new gene therapeutic agent for ovarian carcioma.
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