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The Bladder Cancer-Specificity and Activity of the Expression of Smac Gene Promoted by UPIb Promoter
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Abstract:
Objective: To investigate the bladder cancer-specificity and activity of the expression of Smac gene promoted by UpIb promoter. Methods:Smac mRNA and protein were detected in BIU87 cell line and many other non-bladder cancer cell lines before and after transfection with pcDNA3-UpIb promoter-Smac including UpIb promoter and Smac gene by RT-PCR and immunohistochemical method, respectively.Results:The expression of Smac mRNA in BIU87 cell line increased about 2.1 times after transfection . The Smac protein positive cell rate of BIU-87 cell line was about 25.6% before transfection and it increased to about 70.5% after transfection. There was no significant difference about the expression of Smac mRNA and protein in non-bladder cancer cell lines before and after transfection. Conclusions: UpIb promoter could promote the expression of Smac gene with bladder cancer-specificity and considerable activity, which laid the foundation of target gene therapy for bladder cancer.
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