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Expression and Significance of Vascular Endothelial Growth Factor and Its Receptors (KDR) in Prostate Cancer Cell Lines
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Abstract:
Objective:To investigate the expression of VEGF and its receptor (KDR) in prostate cancer cell lines. Methods: Immunocytochemical staining and Western blot and reverse transcriptase-polymerase chain reaction(RT-PCR)were employed to test the expression of VEGF and its receptor KDR in five human prostate cancer cell lines, LNCaP, PC-3, PC-3M, DU-145, 22-RV1. ELISA was employed to test VEGF concentration in culture supernatant of prostate cancer cell lines. Results:All five prostate cancer cell lines expressed VEGF and KDR. The expression of protein and mRNA in PC-3, DU-145 and LNCaP cell lines was higher than that in PC-3M and 22RV1 cell lines (P< 0. 01). The result of the VEGF concentration in cell culture supernatants is as same as that of immunocytochemical staining.Conclusion: Although they were expressed differentially, both VEGF and KDR were present in prostate cancer cell lines. The expression of VEGF and KDR might be related with the pathogenesis and progress of prostate cancer.
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