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RhoC siRNA inhibits migration and invasion of gastric cancer cells
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Abstract:
To study the effect of RhoC on the metastasis of gastric cancer cells. Methods: A group of RhoC -specific small interfering RNAs (siHNA) were designed and the related vectors were constructed with the assistance of computer. The vectors were transfected into human gastric cancer cell SGC7901 by Iipofectamine2000. The protein expression of RhoC in the translectants was examined by Western blot.Cell growth rate was determined will a Cell Counting Kit -8. MTT assay was used to evaluate the sensitivity of the transfectants to anticancer drugs. Wound-healing and invasion assays were used to examine the abilities of migration and invasion, respectively. Results:Five RhoC-specif-ic siRNAs were designed and the related vectors were constructed successfully. Western blot showed that RhoC-specific siRNA, RhoC-s362, dramatically inhibited RhoC expression. Though down-regulation of RhoC expression did not affect the proliferation and chemo-sensitivity of the transfectants; it did suppress the migrating and invasive abilities of gastric cancer cells. Conclusion: RhoC siRNA RhoC-s362 can effectively inhibit RhoC expression and restrain the migration and invasion of the SGC7901 , suggesting that RhoC play an important role in the metastasis of gastric cancer.
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