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Therapeutic effect of herceptin combined with cDDP on mice receiving implantation of HER-2/neu-overexpression human ovarian cancer
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Abstract:
To establish abdomino-metastasis model of human ovarian carcinoma cell strain SKOV3 (over-expressed HER-2/neu) in nude mice, and to explore the inhibitory effect and mechanism of herceptin combined with cD-D? on mice model. Methods: Ninety-six hours after SKOV3 cell were implanted into abdominal cavity, nude mice were randomly divided into 4 groups: A group normal saline, B group cDDP, C group herceptin and D group herceptin plus cD-DP. The tumor formation rate, survival time and prolongation survival time of tumor-bearing nude mice were evaluated. The changes of Fas and FasL were estimated by flow cytometry( FCM). HER-2/neu in tissue of group A and D was detected with pathological and immunohistochemistry method. Results: HER-2/neu was overexpressed in abdominal cavity implanted with SKOV3 tumors; herceptin combined with cDDP decreased the contents of HER-2/neu. Herceptin and cDDP significantly inhibited abdomino-plantation of SKOV3 in nude mice, and prolonged their survival time. Flow cytometry showed significant difference in apoptosis rate between control group and other groups( P=0. 001 ). The highest apoptosis rate was 55. 9% in the combination group; the expression of FasL was not significantly different between control group and other groups. Conclusion: Herceptin and cDDP can inhibit the growth of SKOV3 cells and prolong the survival duration of tumor-bearing nude mice and the mechanism may be associated with the positive regulation of CD95.
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