Mobile website
Establishment of a mouse lymphopenia model and its immune characteristics
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To establish a mouse lymphopenia model via irradiation and to study its immune characteristics. Methods: Lymphocytes isolated from PBMC, spleen and inguinal lymph-nodes were counted after C57BL/6 mice were treated with irradiation at the dosage of 1. 25 Gy. Subsets of lymphocytes were analyzed by FACS. The expression of transcription factors T-bet and GATA-3 were detected by RT-PCR. Irradiated mice treated with DC-gp100 vaccine were challenged by B16F10 tumor cells, tumor growth was observed. Results: 1.25 Gy irradiation significantly decreased the cell number of lymphocytes, specially the subset of CD4+CD25 +T cells. A memory-like T subset was induced during the course of immune reconstitution. The expression of T-bet but not GATA-3 in the splenocytes was up-regulated. Irradiated mice vaccinated with DC-gplOO could resist the tumor challenge and retard the tumor growth. Conclusion: Lymphopenia mouse model is successfully established and an enhanced immune protection can be induced under the lymphopenia status.
Keywords:
Project Supported:
Clc Number:
