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Influence of RNA interference on expression of Aurora-A in human ovarian cancer cells and on cell proliferation
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Abstract:
Objective: To investigate the inhibitory effect of RNA interference (RNAi) on the expression of Aurora-A in SKOV3 cells and on proliferation of SKOV3 cells. Methods: Two pairs of oligo small interference RNA (Oligo siRNA) specific for Aurora-A were designed for RNAi and were transferred into SKOV3 cells. The expression of Aurora-A were detected by RT-PCR and Western blot. Furthermore, the cell proliferation and apoptosis were observed by MTT and FCM after transfection. Results:After transfection with Oligo siRNA, mRNA and protein level of Aurora-A gene in SKOV3 cells were obviously reduced, while the inhibitory rate of proliferation and apoptosis rate in SKOV3 cells were increased significantly. Conclusion: The Oligo siRNA specific for Aurora-A can reduce the expression of Aurora-A gene and induce apoptosis of SKOV3 cells.
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