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Graft-versus-tumor activity induced by infusion of MHC half-matched bone marrow cells combined with spleen cells in mice bearing H22 tumor
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Abstract:
Objective: To observe the anti-tumor effects of transplanting MHC half-matched bone marrow cells combined with spleen cells in a murine model bearing H22 solid tumor. Methods: Female (BALB/c×C57BL/6) F1 mice (CB6F1，H-2Kb/d) were subcutaneously inoculated with H22 cells to develop a solid tumor model; the model mice were taken as recipients. The syngenic, half-matched, and mismatched donor bone marrow cells were from healthy female F1, male C57BL/6(H-2Kb), and male C3H(H-2K) mice, respectively. The tumor weights were measured 18 days after transplantation. The WBC, biochemistry parameters and the formation of chimerism were observed in mice transplanted with MHC half-matched donor cells irradiated by 7.5Gy 60Co. Meanwhile, graft-versus-host disease (GVHD) was assessed and the results were compared between mice transplanted with radiated and non-radiated donor cells. Results: The tumor weights of mice transplanted with donor cells (radiated/non-irradiated by 60Co) were significantly decreased compared with those of the mice receiving chemotherapy alone（P<0.05）. No anti-tumor effect was found to be induced by MHC half-matched cells in mice receiving no pretreatment of chemotherapy. The transplantation of MHC half-matched donor cells radiated by 7.5 Gy 60Co obviously attenuated GVHD and had no adverse effects on peripheral WBC and functions of liver and kidney. Conclusion:Transplantation of 7.5 Gy 60Co irradiated, MHC half-matched bone marrow cells combined with spleen cells can induce a graft-versus-tumor effect in mice bearing H22 tumor and can attenuate GVHD.
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