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Construction of recombinant fowl poxvirus harboring HN gene of newcastle disease virus and its anti-tumor effect
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Abstract:
Objective: To establish a recombinant fowlpox virus vector harboring HN gene of Newcastle disease virus and to study its inhibitory effect on in vitro cultured human hepatic carcinoma cell line SMMC7721 and on H22 hepatoma in C57BL/6 mice. Methods: The fowl poxvirus 282 E4 was transfected with HN gene of Newcastle disease virus, and the product (vFVHN) was screened by 5-Bromo-2-deoxyribouridine and identified by RT-PCR and Western blotting. The mortality of SMMC7721 cells was detected by Methylthiazoletetra-zolium staining after vFVHN infection ; the tumor suppression rate of vFVHN' on H22 hepatoma in C57BL/6 mice was detected to study its anti-tumor effect. Results:Fowlpox virus harboring HN gene of Newcastle disease was successfully constructed, and its killing effect on SMMC7721 was 43.37% and its anti-tumor rate on SMMC7721 cells was 20.65%. Conclusion: It is demonstrated that vFVHN can effectively kill SMMC7721 cells cultured in vitro and has inhibitory effect on C57BL/6 mice bearing H22 hepatoma.
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