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Effects of dexamethasone on ERK1/2 activity and cyclin D1 expression in human prostate cancer cell line DU145
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Abstract:
Objective: To explore the mechanism by which dexamethasone inhibits androgen-independent prostate cancer cells. Methods: The effects of dexamethasone and RU486, a blocker of dexamethasone receptor, on DU145 cell growth were abserved. Flow cytometric analysis was performed to examine the effects of dexamethasone on cell cycle. Western blot analysis was employed to examine the effects of dexamethasone on extracellular signal-regulated kinase 1/2 activity and cyclin D1 expression in DU145 cells. Expression of glucocorticoid receptor gene mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Dexamethasone dose dependently inhibited the growth of DU145 cell and caused cell arrest at G0/G1 stage. Western blot indicated that ERK 1/2 activity and cyclin D1 expression were suppressed after 3 d culture with dexamethasone. The effects of dexamethasone were blocked by RU486 when added simultaneously with dexamethasone. RT-PCR showed expression of glucocorticoid receptor mRNA in DU145 cells. Conclusion: Dexamethasone can suppress DU145 cell proliferation and cell cycle, possibly through the decreasing ERK1/2 activity and cyclin D1 expression, indicating glucocorticoid may have potential therapeutic effect on prostate cancer.
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