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Expression of anti-γ-seminoprotein single-chain antibody by rapid translation system and determination of its biological activity
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Abstract:
Objective: To prepare the single-chain antibody of γ-seminoprotein and to determine its biological activity and specific targeting ability. Methods: Rapid translation system was used to express single-chain anti-γ-seminoprotein antibody; immunoprecipitation, Western blotting, immunofluorescent staining and cellular test were used to investigate the activity and the efficiency of the antibody after entering into prostate cancer cells. Results: The single-chain anti-γ-seminoprotein antibody was successfully prepared, which could specifically bind with the prostate cells and could bring magnetic beads into prostate cancer cells within 15 min, and the amount of magnetic beads in prostate cancer cells increased as the culture time prolonged. Control study showed that the antibody conjugated magnetic beads could not enter other normal cells or tumors. Conclusion: The prepared single-chain anti-γ-seminoprotein antibody has satisfactory biological activity and targeting ability, which makes it promising for the early NMR detection and targeting therapy of prostate cancer.
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