Mobile website
Inhibitory effects of carbon nanotube-PAMAM-anti-survivin oligonucleotide compounds on proliferation of HepG2 cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the efficiency of carbon nanotube(CNT)-PAMAM mediated entrance of anti-survivin oligonucleotide into HepG2 cells, and its effects on the proliferation of HepG2 cells. Methods: CNT-PAMAM-anti-survivin oligonucleotide compounds were prepared and characterized by AFM and 1% agarose gel electrophoresis analysis. TEM was used to observe the distribution of CNT-PAMAM-ASODN compounds in HepG2 cells. CNT-PAMAM-ASODN compounds were added into the medium and co-cultured with HepG2 cells for 24 h, 48 h,72 h, and 96 h at 37℃, 5% CO2. MTT method was used to detect the effects of ASODN and CNT-PAMAM-ASODN on the proliferation of HepG2 cells. Results: CNT-PAMAM-ASODN compounds were successfully synthesized via AFM and agarose gel electrophoresis. TEM showed that the compounds were located in the cytoplasm. When CNT-PAMAM-ASODN (1.0 μmol/L) and ASODN (1.0 μmol/L) were used for a 48 h culture, the inhibitory rates of HepG2 cells were (45.97±4.28) % for CNT-PAMAM-ASODN compounds group, (9.33±0.85)% for ASODN group, and (6.37±0.69)% for CNT-PAMAM group. CNT-PAMAM-ASODN compounds at 1.5 μmol/L inhibited HepG2 cells by (70.22±7.25)%, and the inhibitory effects were in a time- and concentration-dependent manner. There was statistical difference between experiment group and control group (P<0.01). Conclusion: CNT-PAMAM compounds may serve as a gene delivery vector with high efficiency, which can bring survivin ASODN into HepG2 cells, inhibiting HepG2 cell proliferation and markedly enhancing the inhibitory effects of survivin against HepG2 cells.
Keywords:
Project Supported:
Clc Number:
