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Effect of blocking BRCAA1 gene with siRNA on proliferation of MCF-7 cells and expression of Rb gene
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Abstract:
Objective: To investigate the effect of blocking BRCAA1 gene expression with siRNA on the proliferation of tumor cell line MCF-7 and Rb gene expression. Methods: RNAi was employed to specifically knock down BRCAA1 expression. MCF-7 cells were transfected with complexes constructed with lipids and chemically synthesized Pre-designed anti-BRCAA1 siRNAs. The total RNA was isolated and reversely transcribed after 48 h. The expressions of BRCAA1 and Rb mRNA were determined by Real-Time PCR. Results: Compared with negative control, transfected MCF-7 cells had a 42.3% decrease in expression of BRCAA1 mRNA and an 11.1% increase in Rb mRNA expression. The inhibitory rate of MCF-7 cells proliferation was (81.6±61)%. Conclusion: There may be some antagonistic effect between BRCAA1 gene and Rb gene in proliferation of tumor cells, which provides a potential target for anti-tumor gene therapy.
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