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Sodium butyrate induces apoptosis and regulates p 53 target genes in HT-29 colorectal cancer cells
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Abstract:
Objective: To investigate the regulatory effects of sodium butyrate onp53 target genes (p21waf1, bax,and gadd45) in HT-29 colorectal cancer cells and the related mechanisms. Methods: HT-29 cells were cultured in the absence or presence of sodium butyrate. The cell proliferation and cell cycle were studied by MTT and FCM, respectively. Apoptosis was assessed by observing cell morphology, percentage of sub-G1 cells and AnnexinV-FITC. The effects of sodium butyrate on transcription of p21waf1, bax and gadd45 were analyzed by RT-PCR and Western blot. Results: Sodium butyrate inhibited proliferation and induced apoptosis of HT-29 cells in a time- and dose-dependent manner, and it blocked HT-29 cell at G1 phase. Sodium butyrate stimulated p21waf1 and bax expression both at mRNA and protein level in HT-29 cells, but had little effect on the transcription of gadd45. Conclusion: Sodium butyrate can inhibit proliferation and induce apoptosis of HT-29 cells, which might be through up-regulating p21waf1 and -bax-expression both at mRNA and protein levels.
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