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Mesenchymal stem cells derived from human umbilical cord inhibit activation and proliferation of allogeneic umbilical cord blood T lymphocytes
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Abstract:
Objective: To investigate the effects of human umbilical cord derived mesenchymal stem cells (hUC-MSCs) on the activation and proliferation of allogenic umbilical core blood T lymphocytes, so as to study the immunomodulatory capacity of hUC-MSC. Methods: hUC-MSCs were isolated, culture-expanded from human umbilical cord after enzyme digestion, and the major histocompatibility (MHC) phenotype features of hUC-MSC were detected. The experiment was divided into 3 groups: (1) Negative group: umbilical cord blood MNCs were cultured alone; (2) Control group: MNCs were cultured with nonspecific mitogenic stimuli; (3) Experimental group: MNCs were co-cultured with hUC-MSC and nonspecific mitogenic stimuli. FCM technique was used to detect the CD69 expression, an indicator of T-cell early activation, on T cells and CD4+, CD8+ subsets after 8 hours' co-culture. T-lymphocyte proliferation in each group was detected by MTT after 5 days' co-culture. Results: We successfully established a simple method to isolate and culture-expand abundant hUC-MSCs from human umbilical cord. The immunophenotypic analysis showed that hUC-MSCs expressed no HLA class Ⅱ and less HLA class Ⅰ. hUC-MSC inhibited CD69 expression in PHA and PMA activated T cells from (22.6±5.2)% to (7.8±3.5)%（P<0.01）after 8 hours' co-culture, which influenced both CD4+ and CD8+ T-cell subsets. MTT showed that hUC-MSC dose-dependently inhibited PHA-induced T-lymphocyte proliferation. Conclusion: hUC-MSC has a dose-dependent inhibitory effect on nonspecific mitogenic stimuli triggered activation and proliferation of allogeneic umbilical cord blood T lymphocytes, and the effect is not selective.
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