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Department of Gastroenterology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
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Abstract:
Objective: To study the inhibitory effects of magnetic poly D,L-lactide-co-glycolide phenylarsine oxide nanoparticles (M-PLGA-PAO-NS) on the growth of human hepatic carcinoma cells．Methods： The magnetic polyD,L-lactide-co-glycolide phenylarsine oxide nano-particles were prepared by emulsion-evaporation process. The growth inhibition of tumor cell was studied by the microscope observation and MTT method, and cell apoptosis was observed by FCM．Results: The prepared M-PLGA-PAO-NS had a regular spherical surface and a mean diameter of 290 nm. The magnetic nano-particles effectively inhibited the growth of carcinoma cells (SMMC-7721) in a time- and concentration-dependent manner \[e.g. inhibitory rate of 1.0 μmol/L nano-particles (4.6±0.9)% for 24 h versus (11.4±1.2)% for 48 h \].FCM assay indicated that most of the cells were arrested at S+G2/M phase and there was apoptotic peak . Conclusion： The prepared M-PLGA-PAO-NS has high anti-hepatic carcinoma activity against SMMC-7721, and cells in the G0/G1 phase may be the target for drug action.
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