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Interleukin-24 induced activation and maturation of human dendritic cells
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Abstract:
Objective: To investigate the effects of human IL-24 on phenotype and antigen presenting function of human monocyte-derived dendritic cells in vitro. Methods: Western blot was used to determine the level of IL-24 in the DC culture supernatant.Semi-quantitative RT-PCR was used to analyze the expression of IL-24 receptor and chemokine receptors in DCs at different developmental stages. Fluorescence-activated cell sorting (FACS) analysis was used to assess the expression of MHC class Ⅱ molecule, CD80 and CD86 on DCs surface in the presence of IL-24. Allogenic mixed lymphocyte reaction was employed to analyze the effect of IL-24 on the antigen presenting function of DCs. Results: We found that LPS induced DCs to secrete endogenous IL-24. Meanwhile, LPS also up-regulated the expression of IL-22R1, an IL-24 receptor subunit, on DCs. Supplement of IL-24 to DCs culture medium significantly augmented the expression of MHCⅡ, CD80, and CD86 on DCs surface, and promoted the antigen presenting function of DCs to T cells. Conclusion: IL-24 can promote the activation and maturation of DCs, increasing the antigen presenting function of DCs.
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