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Relationship between adenovirus type 5 infection efficiency and Coxsackie virus-adenovirus receptor on tumor cells
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Abstract:
Objective: To investigate the relationship between adenovirus type 5 infection efficiency and expression of Coxsackie virus adenovirus receptor (CAR) and integrin in different tumor cell lines, providing a basis for further study of adenovirus gene therapy. Methods: The expression levels of CAR in different cell lines were examined by flow cytometry. The cells were infected with Ad5-CMV-GFP and Ad5-CMV-luc carrying report genes, tested the infection efficiency and determined their expression levels by flow cytometry and Luciferase Assay. The variation of adenovirus infection efficiency was evaluated and compared when the CAR expression was enhanced on cell surface with plasmid expressing CAR or blocked by antibodies of CAR and integrin. Results: The highest CAR expression was found in SMMC-7721 and A549 cell lines, the lowest in K562 cells. The results of fluorescence microscope and flow cytometry suggested that Ad5 had the highest efficiency in infecting SMMC-7721 and A549 cell lines, and the lowest in infecting K562 cells. Conclusion：The infection efficiency of adenovirus is determined by the levels of CAR and integrin on cancer cells.
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