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Effect of telomerase antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide
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Abstract:
To observe the effect of telomerase antisense DNA on apoptosis of hepatoma cells induced by arsenic trioxide, in an effort to look for a new anti-hepatic cancer agent with high efficiency, low cytotoxicity. Methods: We designed and synthesized a 20nt telomerase-antisense DNA targeting telomerase template and observed its influence on the telomerase activity of hepatoma cells. H-E staining, flow cytometry, and DNA agarose electrophoresis were used to study the preventive effect of telomerase-antisense DNA on hepatoma cells apoptosis induced by arsenic trioxide. Flow cytometry was used to detect the expression of Fas, Fas-L, and bcl-2. Results: Telomerase-antisense DNA (5 μmol/L) effectively inhibited the telomerase activity of hepatoma cells after 24 hours (P<0.01). When telomerase activity was inhibited by telomerase-antisense DNA, the hepatoma cells became more sensitive to arsenic trioxide induced apoptosis, which was induced through Fas and Fas-L pathway. Conclusion: Inhibition of telomerase activity can obviously increase the apoptosis rate of hepatoma cells induced by arsenic trioxide, therefore reducing the quantity of the latter in treating HCC, indicating a future for the combined application of both in clinical practice.
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