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Effects of natural killer cells with killer immunoglobulinlike receptors incompatibility on breast cancer cells
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Abstract:
To study the effects of natural killer cells with KIR(killer immunoglobulinlike receptor)/HLACw incompatibility on breast cancer cell lines in vitro, and to analyze the relationship between NKG2D (on NK cells) and MICA (on tumor cells) expression with NK cells alloreactivity against breast cancer.Methods: Monocytes were derived from 10 ml peripheral blood of healthy donors and patients of breast cancer by FicollHypaque. NK cells were purified by MASC separation system with NK Cell Isolation Kit. The polymerase chain reactionbased sequencespecific primer (PCRSSP) was used for KIR typing of NK cells and HLACw typing of tumor cells. NK cell cytotoxicity was assessed by MTT assays. Flow cytometry was used to investigate the expression level of NKG2D in NK cells and RTPCR was used to investigate the expression MICA in tumor cells. Results: The purity of enriched NK cells was more than 90% as determined by flow cytometry. The cytotoxicity of NK cells in KIR/HLACw compatible group was greatly higher than that of incompatible group. The cytotoxicity rates of NK cells of G1 and G2 group against MDAMB435s（G1 group）were (84.6±16.7)% and (25.4±8.9)%, respectively; against SKBr3（G1 group） were (70.2±11.8)% and (29.5±6.8)%, respectively; against MCF7（G2 group）were (33.1±5.7)% and (81.4±10.3)%; respectively. MICA expression was noticed in MCF7, MDAMB435s, and SKBr3 breast cancer cell lines. The expression of NKG2D on the NK cells was increased when cocultured with MCF7 cells. Conclusion: The cytotoxicity of NK cells against breast cancer cells is not mediated by KIR/HLACw incompatibility. MICA might increase the sensitivity of the breast cancer to NK cells by activating NKG2D on the NK cells.
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Clc Number:
R730.3
