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Antitumor effects of pIRApoptinHNIL18 on colon carcinoma cell HCT116
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Abstract:
To investigate the combined antitumor effects of apoptin gene, hemagglutininneuramidinase gene (HN) of newcastle disease virus (NDV), and human IL18 gene(hIL18) on colon carcinoma cells HCT116in vitro. Methods:The recombinant plasmid pIRApoptinHNIL18 was introduced into human colon carcinoma cells HCT116 by liposomemediated transfection. The expression of the exogenous genes was detected by Western blotting and RTPCR. The antitumor effects of pIRApoptinHNIL18 on HCT116 cells were determined by AO/EB staining. The influence of pIRApoptinHNIL18 on mitochondrial transmembrane potential (△Ψ) and reactive oxygen species (ROS) were detected by flow cytometry (FCM). Sialic acid content was determined by using 3, 5dihydroxytoluene. Results: pIRApoptinHNIL18 transfection resulted in expression of the exogenous genes, repression of HCT116 cells, downregulation of △Ψ \[(94.41±8.17) % vs (30.70±8.01)%,P<005\] and Sialic acid content \[(0.33±0.06) mmol/L vs (0.09±003) mmol/L,P<0.01\], and upregulation of ROS \[(52.48±6.09)% vs (68.98±7.26)% P<0.05\]. Conclusion: pIRApoptinHNIL18 may inhibit the growth of HCT116 cells via mitochondrial pathwayinduced apoptosis.
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Clc Number:
R719.31
