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Therapeutic effect of replicationdeficient adenovirus carrying p16 gene on gastric cancer xenografts in nude mice
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Abstract:
To construct a replicationdeficient adenovirus carrying p16 gene and to investigate its antitumor activity on human gastric cancer xenografts in nude mice. Methods: p16 cDNA was amplified by PCR and was inserted into the plasmid pSuCMV, the latter was then used to recombine the replicationdeficient adenovirus AdCMVp16 in 293 cells. Human SGC7901 gastric cancer xenograft models were established in nude mice and were divided into 3 groups: AdCMVp16, AdLacZ, and control groups. Mice in AdCMVp16 group received intratumoral injections of 2×10 8 pfu/100 l AdCMVp16 ( injected every other day for 5 times). Mice in control group received the same volume of virus preserving solution. The tumor volumes were measured at predefined time points. The antitumor effect of AdCMVp16 was observed by p16 immunochemical study and TUNEL detection of cell apoptosis. Results:The replicationdeficient adenovirus expressing p16 gene evidently inhibited the growth of human gastric cancer xenografts in nude mice (P<0.01), with the inhibition rate being 58.12%; but AdLacZ showed no obvious effect on the growth of xenografts (P>0.05), only with a inhibition rate of 4.26%. The pathological examination showed that apoptoses were the main changes in AdCMVp16 group, and p16 gene was found in the cancer cells. Conclusion: The replicationdeficient adenovirus harboring p16 gene can recover the expression of p16 in gastric cancer cells and subsequently inhibit the growth of human gastric cancer.
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Clc Number:
R730.53
