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A functional antibody targeting lung cancer and it′s antigen
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Abstract:
To identify a functional monoclonal antibody 1E2 against lung cancer and its antigen, so as to provide a candidate antibody drug and molecule target for the anti-lung cancer therapy. Methods: The inhibition of the lung cancer cells, binding with tumor cells, isotypes, and inhibition of implanted tumor growth of 1E2 monoclonal antibody were studied by immunofluorescence, ELISA, cell proliferation assay, and in vivo tumor treatment experiments. The corresponding antigen of 1E2 was identified by Western blotting and MALDI-TOF mass spectrometry. Results: The 1E2 antibody could recognize cell surface protein of lung cancer cell line GLC82 and HCI-H520 and obviously inhibited the lung cancer cell growth in vivo. Animal experiment revealed that 1E2 antibody significantly inhibited lung tumor growth by 49%. Western blotting revealed that the molecular weight of the antigen of 1E2 antibody was about 110 000. Mass fingerprint revealed this protein was carbamoyl-phosphate synthetase 1(CPS1). Conclusion: 1E2 monoclonal antibody can suppress lung tumor growth in vitro and in vivo; it might become a candidate drug for targeted lung cancer treatment. CPS1, the antigen of 1E2, locates on the membrane of lung cancer cells and it may become a novel molecule target for lung caner therapy.
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