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Inhibitory effect of recombinant human adiponection on invasion and metastasis of breast cancer cells in vitro
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Abstract:
To investigate the effects of recombinant adiponectin (RA) on the invasive ability of human breast cancer cell line MDAMB231. Methods: MDAMB231 cells were divided into control group and RA(2.5, 5.0, 10, 20, and 30 μg/ml) treatment groups. The effect of RA on proliferation of MDAMB231 cells was measured by MTT assay; the invasive and migration abilities of MDAMB231 cell were assayed in Transwell cell culture chamber; cell adhesion assay was carried out in a microculture well precoated with fibronectin．Gelatinolytic activities of both MMP2 and MMP9 secreted by cancer cells were measured by zymogrophy analysis. Results: The growth and invasive ability of MDAMB231 cells were obviously inhibited when the concentration of RA was higher than 5.0 μg/ml (P<0.01); the inhibition of migration was in a concentrationdependent manner, with the inhibitory rate ranging from 22.64％77.84％. When the concentration of RA was higher than 2.5 μg/ml, the migration of tumor and the secretion of MMP2 and MMP9 were obviously inhibited (P<0.05,P<0.01); the secretion of MMP9 decreased with the increase of RA concentration. RA was more sensitive to FN and than to ECM. Conclusion: These results indicate that adiponectin is effective in suppressing tumor cell invasion when its concentration is >2.5 μg/ml; the mechanism might be the downregulation of MMPs.
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