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shRNA interfering human etherαgogorelated potassium channel in treatment of subcutaneously implanted human neuroblastoma SHSY5Y cells in nude mice
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Abstract:
To explore the therapeutic efficacy and mechanism of shRNA in treatment of subcutaneously implanted SHSY5Y cells in nude mice by interfering human etherαgogorelated gene （HERG）potassium channel expression. Methods: Eukaryotic vector mU6pro was used to construct herg genetargeting small hairpin interfering RNA plasmid: shRNAherg. Eighteen BALB/c nude mice were inoculated with SHSY5Y cells subcutaneously to establish neuroblastoma model. When the implanted tumors grew to about 100 mm3, the mice were divided into randomly 3 groups: Intratumor injection of normal saline (group Ⅰ), shRNAcontrol plasmid (group Ⅱ), and shRNAherg plasmid (group Ⅲ). The injections were given twice at an interval of 7 d after the first injection. The tumor growth was observed; the expression of mRNA and protein of HERG potassium was determined by RTPCR and the immunohistochemical method, respectively. Results: The tumor growth was obviously inhibited in group Ⅲ; the inhibitory rates in groupⅡand group Ⅲ were 3.04% and 29.78%, respectively (P< 0.05). Compared with groupⅠandⅡ, group Ⅲ had lower levels of HERG mRNA and protein after treatment. Conclusion: The constructed shRNA can depress the growth of neuroblastoma by specifically silencing the expression of HERG potassium protein.
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