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Expression of histone deacetyase 4 in human liver carcinoma cell line Bel7402 and its significance
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Abstract:
To investigate the expression of histone deacetyase4 (HDAC4) in human liver carcinoma cell line Bel7402 and to explore the regulatory effects of HDAC4 on the proliferation and differentiation of Bel7402. Methods: Carcinoma cells Bel7402 was treated with different concentrations of sodium phenylbutyrate (SPB), an inhibitor of HDAC4. Expression of HDAC4 mRNA in Bel7402 cells was analyzed by RTPCR before and after SPB treatment. Reverse microscope was used to observe the morphological changes of Bel7402 cells. MTT assay and flow cytometry were adopted to describe the proliferation and cell cycle of Bel7402 cells. Expression of P27 protein was determined by immunohistochemical method. The statistical analysis was performed using oneway ANOVA and student t test. Results: SPB significantly decreased the expression of HDAC4 in Bel7402\[(0.88±0.13) vs (0.12±0.04), P<0.05\]. It also inhibited the Bel7402 cell growth in a timeand dosedependent manner. Fibrous changes of Bel7402 cells was observed after SPB treatment. SPB treatment arrested cell at G1 phase \[(50.6±4.0)% vs (78.8±3.6)%, P<0.05\] and enhanced the expression of P27 in Bel7402 \[（23±11) vs (61±7),P<0.05\].Conclusion: SPB treatment can decrease the expression of HDAC4 in human liver cancer cell Bel7402 and subsequently inhibits proliferation of Bel7402 cells, which might be associated with the change of P27 protein expression.
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