Mobile website
Influence of adenovirus mediated PTEN gene on growth of A549 cells in vitro and in vivo
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To study the inhibitory effect of adenovirus mediated (phosphatase and tensin homologuedeleted chromosome ten gene) PTEN gene on the growth of A549 cells in vitro and in vivo. Methods: The PTEN gene was amplified by RTPCR from human peripheral blood and was inserted into pAdTrackCMV to construct pAdTrackCMVPTEN. The growth inhibition effect of pAdTrackCMVPTEN on A549 cells was detected by MTT; flow cytometry was used to detect the cell cycle and apoptosis of the tumor cells. The tumor inhibitory effect of pAdTrackCMVPTEN on A549 cells was studied with tumorbearing mice model. The microvessel density (MVD) of the implanted tumor tissue was determined immunohistochemically. Results: The sequencing result of cloned PTEN accorded well to that in GeneBank. The apoptosis rate of A549 cells was 10.5% 48 h after infection with pAdTrackCMVPTEN, significantly higher than that in the control group (0). Four days after infection, the growth of the A549 was inhibited by 57%. At the end of tumor inhibition experiment, the average tumor weight was (0.58±0.29) g in pAdTrackCMVPTEN treated group and (1.42±0.24) g in the control group, with a inhibitory rate of 59%（P<0.05）; pAdTrackCMVPTEN also decreased MVD by 49%（P<005）. Conclusion: We have sucessfully constructed pAdTrackCMVPTEN, which can inhibit the growth of A549 cells in vitro and in vivo.
Keywords:
Project Supported:
Clc Number:
