Mobile website
Survivin targeted siRNA inhibits proliferation of human osteosarcoma cell line MG63 in vitro and in vivo
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To explore the inhibitory effect of survivin targeted siRNA on human osteosarcoma cell line MG63 in vivo and in vitro. Methods: According to the survivin cDNA sequence, the specific RNA interference (RNAi) fragments were designed and synthesized, which were then cloned into pSilencer 3.0 H1 neo plasmid vector to construct pSiS vector. MG63 cells were transfected with RNAi vectors and negative control vector separately; the stably transfected cell strains were selected by G418; and cell growth was assessed by cell counting. Expression of survivin protein was investigated by Western blotting. Apoptosis analysis was examined by AnnexinⅤ method. Tumorigenesis assay was conducted in nude mice. Results: The specific survivin targeted siRNA eukaryotic vector pSiS was successfully constructed, and transfectants were obtained. Compared with blank vector transfected cells and un transfected cells, MG63/pSiS cells had a slow growth ( P <0.01). Expression of survivin protein was significantly inhibited in MG63/pSiS cells, which had an increased apoptotic rate ( P <0.01). The tumorigenesis of transplanted MG63/pSiS was greatly inhibited. Conclusion：Survivin targeted siRNA can promote the apoptosis of MG63 cells, and inhibit the in vitro growth and in vivo tumor formation in nude mice.
Keywords:
Project Supported:
Supported by the National Natural Science Foundation of China (No. 30471988)
Clc Number:
