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Bifidobacterium exopolysaccharide loaded nanoparticles induces apoptosis of human gastric cancer cells transplanted into nude mice
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Abstract:
To prepare Bifidobacterium exopolysaccharide loaded nanoparticles（ B. EPS NPs）and to investigate the effect of B. EPS NPs on the proliferation and apoptosis of human gastric cancer cells transplanted in nude mice. Methods: B. EPS and nanoparticles of Fe3O4 were used to prepare B. EPS NPs by the method of emulsion polymerization. Transplant models of human gastric cancer cells BGC 823 were established in nude mice and were divided into 5 groups after 6 days, namely, the normal saline group, nanoparticle group(NPs), cytoxan (CTX) group, B. EPS group, and B. EPS NPs group. The corresponding agents were used for gastric lavage. The growth of tumor was observed and cell apoptosis was detected by TUNEL. The ultra microstructure of tumor cells was observed under TEM, expression of proli ferating cell nuclear antigen (PCNA), bcl 2 and bax was examined by immunohistochemistry method. Results: The diameters of the empty loaded NPs and B. EPS NPs were (560±21）nm and （960±32）nm, respectively; the drug loading capacity of B. EPS was 30%. The growth of the transplanted tumor was inhibited in B. EPS NPs group, with the inhibitory rate being （46.4±2.94）%, which was higher than that of the B. EPS group(\[320±1.62\]％) and NPs group \[(4.9±0.98\]％). The apoptosis index in B. EPS NPs group (\[66.8±5.58\]％) was significantly higher than that of the B. EPS group（ \[41.3±4.26\]％, P <001）. TEM showed typical apoptotic structures in B. EPS NPs group. Compared with B. EPS group， B. EPS NPs group had markedly lower expression of PCNA and bcl 2 ( P <001) and higher expression of bax ( P <0.01). Conclusion: Nanoparticles can enhance the inhibitory effect and pro apoptosis effect of B. EPS for human gastric cancer (BGC 823) transplanted tumor.
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Supported by the National Natural Science Foundation of China (No.30371055)
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