Mobile website
Culture and identification of endothelial progenitor cells from cord blood with CD133 immunomagnetic sorting
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To establish a method for isolating and culturing endothelial progenitor cells（EPCs), which have high potential of proliferation, migration and angiogenesis, from cord blood. Methods: CD133 +cells were selected from fresh cord blood mononuclear cells by magnetic activated cell sorting system (MACS), and were cultured in EGM 2MV medium. EPCs were identified by examining the morphology, cell markers and functions. And the EPCs were compared with human umbilical vein endothelial cells (HUVECs). Results: On the 7 th day, the adherent cells exhibited the small colonies; and on the 21 th day,the colonies were expanded，confluenced and displayed a typical “cobblestone” morphology. On the 14 th day, 90% attached cells took up Dil ac LDL, and bound FITC UEA 1 (double positive fluorescence). The cell markers of CD133 and CD34 decreased from 86.04% to 2.96% and 90.88% to 2.99%, respectively, while CD31 increased from 1.12% to 99.88%. Compared with HUVECS, EPCs had more potent potential of proliferation, migration and angiogenesis( P <0.05). Conclusion: CD133 + MACS can be used to isolate EPCs, with high capacity of proliferation, angiogenesis and migration, from cord blood mononuclear cells.
Keywords:
Project Supported:
Supported by the Medicine Science and Technology Development Foundation of Jiangsu Health Department (No.H200510)；the Medical Major Talent Program of Jiangsu Province（No.RC2007061）
Clc Number:
