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Hedgehog signaling pathway regulates growth of human digestive tract tumor cells
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Abstract:
To study the expression of Shh, patched(PTCH), smoothened (Smo) and Gli 1 genes，four components of the Hedgehog signaling pathway, in colonic cancer cell lines, pancreatic cancer cell line and colonic adenoma tissues, and to discuss the influence of cyclopamine, a Smo receptor specific inhibitor, on the growth of these tumor cells. Methods: The expression of Shh, PTCH, Smo and Gli 1 were investigated using RT PCR in 4 colonic cancer cell lines (LS174T, HCT116, SW116 and CT26), pancreatic cancer cell line BxPC3 and 2 colonic adenoma tissues. MTT method was used to study the inhibitory effect of cyclopamine on the growth of these cancer cell lines in vitro . Results: Shh, PTCH, Smo and Gli 1 genes were expressed in 2 of colonic adenoma tissues and SW116, CT26 and BxPC3 cells. The mRNA of Smo and PTCH genes were not found in LS174T and HCT116 cells; the expression of Shh and Gli 1 mRNA were significantly up regulated. Cyclopamine inhibited the growth of SW116, CT 26 and BxPC3, expecially the positive cells of Smo gene. Conclusion: Shh, PTCH, Smo and Gli 1 genes are expressed in colonic cancer cell lines, pancreatic cancer cell line and colonic adenoma tissues, Cyclopamine has obvious inhibitory effect on cells with overexpression of Smo , which implies that Hedgehog signaling pathway might be activated in some tumor cells of digestive system.
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