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Inhibitory effects of adenovirusmediated biantisense ornithine decarboxylase and s-adenosylmethionine decarboxylase on proliferation and invasion of esophageal cancer cells
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Abstract:
Abstract Objective: To study the inhibitory effects of AdODCAdoMetDCas, a recombinant adenovirus with biantisense RNA of ornithine decarboxylase (ODC）and sadenosylmethionine decarboxylase（AdoMetDC）, on cell proliferation and invasion of esophageal cancer cells. Methods: The AdODCAdoMetDCas was used to infect esophageal cancer Eca109 cells. Western blotting was used to examine the protein expression of ODC and AdoMetDC in Eca109 cells. The content of polyamine in Eca109 cells was determined by HPLC. The inhibition on the proliferation of Eca109 cells was investigated by viable cell counting. Matrigel invasion assay was used to study the invasion ability of Eca109 cells. Furthermore, the antitumor effect of AdODCAdoMetDCas was evaluated in a nude mouse xenograft model. Results: Western blotting assay showed that AdODCAdoMetDCas inhibited the expression of ODC and AdoMetDC; HPLC indicated that the contents of putrescine, spermidine and spermine were markedly decreased in Eca109 cells after infection with AdODCAdoMetDCas（P<0.01）. Viable cell counting showed that AdODCAdoMetDCas significantly inhibited the proliferation of Eca109 cells（P<0.01）. Matrigel invasion assay showed that AdODCAdoMetDCas significantly decreased the invasion ability of Eca109 cells in vitro(P<0.01). Meanwhile, experiments with nude mouse xenograft model demonstrated that AdODCAdoMetDCas had significant antitumor ability in vivo （P<0.05, P<0.01）. Conclusion: AdODCAdoMetDCas can significantly inhibit the proliferation and invasion of esophageal cancer, which makes it a potential therapy for the treatment of esophageal cancer.
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Project Supported:
Supported by the National Natural Science Foundation of China (No.30571844); Postdoctoral Foundation of Shandong University (2005)
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