Mobile website
Inhibitory effects of 5Aza2′deoxycytidine and trichostatin A against human endometrial cancer cell line Ishikawa
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Abstract Objective: To study the inhibitory effect of demethylating agent 5Aza2′deoxycytidine（5AzaCdR）and trichostatin A (TSA) against human endometrial cancer cell line Ishikawa in vitro. Methods: Ishikawa cells were treated with 5AzaCdR, TSA or a combination of both and the growth curves of cells in each group were obtained by trypanblue exclusion assay and cell counting. The cell apoptosis was examined by Annexin V, cell cycle by flow cytometry, and cleavage of poly ADPribose polymerase (PARP) and activation of caspase8 by Western blotting. The effect of the 2 inhibitors on the survival rates of transplanted tumors, tumor sizes and weights were studied in mouse transplantation model of Ishikawa cells. Results: 5AzaCdR and TSA inhibited the growth of Ishikawa cells in a dose and timedependent manner. 5AzaCdR combined with TSA resulted in greater growth inhibition than they were used alone（P<0.05）. 5AzaCdR and TSA both induced apoptosis of Ishikawa cells, and a combination of the two caused higher apoptosis rate（P<0.05）. 5AzaCdR combined with TSA also induced the G1 phase arrest of Ishikawa cells. Caspase8 activation and cleavage of PARP were confirmed by Western blotting. 5AzaCdR and TSA inhibited the growth of transplanted tumors and reduced the volume and weight of tumors. Conclusion: 5AzaCdR and TSA can inhibit the growth of Ishikawa cells and induce their apoptosis, and combination of both has more potent effect, which might be related to the activation of caspase8 and the cleavage of PARP.
Keywords:
Project Supported:
Supported by the Initate Overseas Intelligence Program Foundation of Shandong Province (No.20083700340)
Clc Number:
