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Tumor suppressor gene OVCA1 inhibits migration and invasion of ovarian cancer cells A2780 in vitro
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Abstract:
Abstract Objective:To investigate the inhibitory effects of tumor suppressor gene OVCA1, also named diphthamide synthesis protein 2like(DPH2L), on migration and invasion of ovarian cancer cell A2780 in vitro. Methods: Ovarian cancer cell line A2780 was transfected with GFPtaggedOVCA1 vector or empty vector via Lipofetamine 2000. Cells stably expressing GFPtaggedOVCA1 (A2780OVCA1) or GFP (A2780GFP) were screened by G418. Stably transfected monoclones were obtained by limiting dilution. GFP protein in the cells was examined by fluorescence microscopy and OVCA1 fragment was examined by RTPCR. Wound healing assay, transwell migration assay and matrigel invasion assay were employed to study the effects of OVCA1 on A2780 cell migration and invasion, while taking A2780GFP and A2780 cells as controls. Results:The cell line stably expressing GFPtagged 〖STBX〗OVCA1〖STBZ〗 (A2780OVCA1) was successfully established. The migration speed of cells in A2780OVCA1 group was significantly lower than those of the other 2 groups (P<0.05). Cells passing the pores of transwell filter was less in A2780OVCA1 cells than in the cells of the 2 control groups (P<0.05). Cells passing matrigelcoated filter was significantly decreased in A2780OVCA1 group than in the 2 control groups (P<0.05). Conclusion: OVCA1 can effectively inhibit A2780 cell migration and invasion in vitro.
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Project Supported:
Supported by the Foundation from the Educational Department of Liaoning Province (No.2004F053)
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