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Inhibitory effect of resveratrol on human gastric cancer cell line SGC7901 and the possible mechanism
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Abstract:
Abstract Objective:To investigate the inhibitory effect of Resveratrol (Res) on human gastric cancer cell line SGC7901 and the related mechanism.Methods： Human gastric cancer SGC7901 cells were treated with different concentrations of Res（10, 20, 40 μg/ml）; cells treated with DMSO and culture medium served as controls. The growth inhibition rate of SGC7901 cells was examined by MTT assay; the morphological changes of cells were observed under phasecontrast microscope; caspase3 activity was assessed by colorimetry; and the cell cycle was detected by flow cytometry(FCM). Results: Res inhibited the growth of SGC7901 cells in a time and dosedependent manner, with maximal inhibitory rate being (53.39±5.32)%. After Res treatment the amount of suspended cell increased and the cells shrank, became round and smashed, with particlelike substance found in the cells. The most obvious changes were found 48 h after treatment with 40 μg/ml Res. Besides, Res upregulated the Caspase3 activity in SGC7901 cells in a time and concentrationdependent manner. Flow cytometry revealed that Res induced the S phase arrest of SGC7901 cells. Conclusion：Res can obviously inhibit the growth of human gastric cancer SGC7901 cells, possibly through activating Caspase3,inducing cell apoptosis and influencing cell cycle.
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Project Supported:
Supported by the Education Commission Foundation of Heilongjiang Province (No.11521282)
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