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cDNA microarray technique in screening primary drug resistancerelated genes of human malignant glioma
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Abstract:
Abstract Objective: To screen for primary drug resistancerelated genes of human malignant glioma using cDNA microarray, so as to provide evidence for drugsensitive predicting gene for chemodrugs for malignant gliomas of different patients. Method: Six fresh glioma specimens were obtained immediately after surgical resection and were primary cultured. MTT method was used to determine the inhibitory effect of Teniposide (VM26) against gliomas. When 4.5 μg/ml（peak blood drug concentration, 1PPC）was used, the inhibitory rate >30% was considered sensitive and the rate ≤30% was considered resistant; and the 6 specimens were divided into 2 group according to the above standard. cDNA microassay combined with clustering analysis was used to screen for resistancerelated genes. Semiquantitative RTPCR was used for verification of HDAC1 gene expression. Results: Three of the 6 glioma specimens belonged to the drug resistance group and the other 3 to the drug sensitive group. cDNA microarray analysis combined with cluster analysis screened out 21 genes, with 6 upregulated and 15 downregulated. High expression of gene HDAC1 was noticed in all the 6 specimens by semiquantitative RTPCR, and the trend was similar to that by microassay. Conclusion: The primary drug resistance of glioma may be associated with the 21 genes screened by cDNA microarray; the detailed mechanisms for drug controlling still need to discussed in the future.
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Supported by the Science and Techology Development Program of Tianjin (No.033182911)
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