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Expression of MCM4 and CDC6 in uterine cervical carcinoma and its relation with HPV16/18 infection
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Abstract:
Abstract Objective: To investigate the expression of minichromosome maintenance proteins 4(MCM4) and cell division cycle 6 (CDC6) in uterine cervical carcinomas and its relationship with human papilloma virus (HPV)16/18 infection. Methods：The expression of MCM4 and CDC6 was examined in 50 squamous cell carcinoma specimens, 20 cervical intraepithelial neoplasia (CIN)ⅡⅢ specimens, 20 CINⅠ specimens, and 20 normal cervical tissues by immunohistochemical method. The infections of HPV type 16, 18 DNA were determined by PCR. Results：(1) The expression of MCM4 and CDC6 in uterine cervical carcinoma tissues was significantly higher than that in the CIN specimens and normal cervical tissues (Both P<0.05). Expression of MCM4 and CDC6 was correlated with tumor grades and lymph node metastasis (all P<0.05), but not with age and clinical stages (all P>0.05). (2) The positive rates of (HPV)16/18 were significant different between cervical carcinomas, CIN and normal tissues (P<0.05), and was not associated with age, tumor grades, clinical stages or lymphnode metastasis (all P>0.05). (3) MCM4 expression were positively correlated with the expression of CDC6 in uterine cervical carcimonas（r=0.390, P<0.05）. The positive rate of HPV16/18 was positively correlated with the expression of MCM4 and CDC6 （r= 0.634, P<0.05; r=0.386 P<0.05）.Conclusion: The results suggest that changes in MCM4 and CDC6 expression in CIN and cervical carcinoma specimens are associated with HPV16/18 infection, which is related to the progression of CIN and carcinogenesis of cervical carcinomas.
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