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Realtime quantitative PCR in determination of VEGF expression in endometrial carcinoma and peripheral blood and its clinical relevance
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Abstract:
Abstract Objective: To examine the expression of VEGF gene in the endometrial carcinoma tissues, paratumor tissues, normal endometria and peripheral blood, and analyze the role of VEGF in tumor growth and tumor metastasis. Methods: Realtime fluorescence quantitative PCR was used to detect the expression of VEGF gene in 51 endometrial carcinoma samples and the corresponding paratumor tissues, 40 normal endometria samples and their corresponding peripheral blood samples. The relation between the VEGF expression and clinical pathological parameters was analyzed.Results: The expression of VEGF gene was higher in the endometrial carcinoma tissues than in the corresponding paratumour tissues and normal endometrial tissues (P<0. 05) . VEGF expression in endometrial carcinoma was significantly correlated with the clinical stage, histological grade, lymph node metastasis and depth of myometrial invasion (all P<0.05), but not with the presence of menopause or the pathological types of the tumor (P>0.05). The expression of VEGF in peripheral blood was higher in patients with endometrial carcinoma than that in the normal controls; and the expression was significantly correlated with the clinical stage, histological grades, pathological types and the presence of lymph node metastasis (P<0.05), but not with the depth of myometrial invasion and the presence of menopause( P>0.05). Conclusion: Realtime fluorescent quantitative PCR can sensitively, specifically detect the expression of VEGF in the endometrial carcinoma tissues and peripheral blood samples. VEGF might play an important role in the development, invasion, and metastasis of endometrial carcinoma.
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Project Supported:
Surpported by Science and Technology Foundation of Qingdao (No.051NS77)
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