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Expression of extracellular matrix protein 1 in breast cancer tissue and cell lines and its significance
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Abstract:
Abstract Objective: To investigate the expression of extracellular matrix protein 1 (ECM1) in breast cancer tissues and cell lines and to assess its significance. Methods: Immunohistochemical and immunocytochemical EnVision methods were used to detect expression of ECM1 in the breast tissues（normal tissues and cancer tissues） and in breast cancer cell lines of different malignancies ( MCF7，SkBr3，and MDA435). The ECM1 positive rates were compared between normal tissues and cancer tissues and between cancer tissues with and without lymph node metastasis. Western blotting was used to identify the subtypes of ECM1. Results: The positive rate of ECM1 was 8.7%(2/23) in the normal breast tissues, 60.4%(29/48) in breast cancer tissues, 38.5% (10/26) in breast cancer tissues without lymph node metastasis, and 864% (19/22) in those with lymph node metastasis. Statistical analysis indicated that the ECM1 expression was higher in the breast cancer tissues than in the normal tissues (P<0.01), and the expression in the metastatic breast cancer was higher than that in the primary cancer tissues (P<0.01). Cell lines with low malignancy, such as MCF7 and SkBr3 cells, were negative of ECM1; cell lines with high malignancy, such as MDA435 cells, were strongly positive of ECM1. Western blotting showed that the highly expressed ECM1 protein was mainly ECM1a subtype, with a relative molecular mass of 68 000. Conclusion: ECM1, mainly ECM1a subtype, is overexpressed in breast cancer tissues and cell lines of high malignancy, which is associated with the metastasis of breast cancer.
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Project Supported:
Surpported by the National Natural Science Foundation of China(No. 30080027); the Major Basic Research Program Foundation of Shanghai of China（No.02JC14005）; the Science and Technology Research Project of Songjiang District of Shanghai（No.06KG27）
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