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Nanocomoposite probes composed of fluorescent magnetic nanoparticles and PSA ScFv antibody for targeted imaging and therapy of prostate cancer
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Abstract:
Abstract Objective: To investigate the feasibility of targeted imaging and therapy of prostate cancer using nanocomposite probes composed of fluorescent magnetic nanoparticles (FMCNPs) and single chain Fv (ScFv) antibody specific for gamaseminoprotein. Methods: The nanocomposite probes (FMCNPsScFv) were prepared by conjugating fluorescent magnetic nanoparticles with singlegamachain Fv antibody specific gamaseminoprotein, and were characterized by high resolution transmission electron microscopy, fluorescent spectrum and magnetic spectrum. Nanocomposite probes were incubated with prostate cancer LNCaP cells, and the targeting results of nanocomposite probes were observed by fluorescent microscopy. The cytotoxicity effect of the nanocomposite probes was measured by MTT. Nude mice models of prostate cancer were established and identified by immunohistochemistry method. The nanocomposite probes were injected into nude mice via tail vein. The distribution of nanocomposite probes in the nude mice was observed by Microanimal imaging system, targeted imaging of the prostate cancer was observed by MR instrument. The nude mice with prostate cancer were irradiated with 100 W magnetic field for 30 min, and the changes of tumor sizes were observed. Results: The FMCNPsScFv nanocomposite probes were successfully prepared. Nanocomposite probes entered into the cytoplasm of cancer cells and exhibited low cytotoxicity effect. Nude mice model with prostate cancer were successfully fabricated; the nanocomposite probes distributed quickly in the main organs of mice, and gradually concentrated on the tumor tissues within 24 h. MR images showed that the tumor images were gradually enhanced from 6 h to 24 h after injection of the nanocomposite probe. Four days after magnetic irradiation, the tumors in the nude mice grew slower compared with the control nude mice (P<0.05).Conclusion:The nanocomposite probes composed of FMCNPs and ScFv antibody can be used for targeted imaging of prostate cancer by MRI instrument and for therapy under in vitro magnetic field.
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Project Supported:
Supported by the Major State Basic Research Development Program (973) of China (No.2005CB723400G); the National High Tecnology Research and Development Program (863) of China (No.2007AA022004); the National Natural Science Foundation of China(No.30771075;No.30672147)
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