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Regulation of RPL4 promoter activity by transcription factor Runx1
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Abstract:
Abstract Objective: Previous genomewide microarray analysis found TTCATTCT motif （DNA binding site of Runx1）in the RPL4 promoter of childhood leukemia cells, suggesting that Runx1 may regulate the expression of RPL4. This study is to investigate the relationship between Runx1 and RPL4 so as to better understand the effects of Runx1 on RPL4 gene transcription, laying a foundation for further studying leukemogenesis of childhood leukemia. Methods: The luciferase plasmids containing RPL4 promoter and its mutant were constructed and were cotransfected into 293T cells with the expression plasmid of Runx1. The transactivity of RPL4 promoter was assayed by luminometer. Results: Runx1 significantly decreased the transcriptional activity of RPL4 promoter (P<0.01), and the decreasing degree was associated with the increase of Runx1 dose (P<0.01). Runx1 protein had no significant effect on the transcriptional activity of RPL4 promoter mutant (P>0.05). Conclusion: Runx1 can inhibit RPL4 gene transcription in a dosedependent manner through binding to the TTCATTCT motif.
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Project Supported:
Supported by the National High Technology Research and Development (863) ProgramBioTec Project (No. 2006AA02Z4Z2); Beijing Excellent Talents Support Program (No. 2004 1D 0300830)
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